In this article we will discuss about the Polymerase Chain Reaction:- 1. Steps of Polymerase Chain Reaction 2. Advantages of Polymerase Chain Reaction 3. Applications. Steps of Polymerase Chain Reaction: PCR uses DNA polymerase to amplify repetitively targeted portions of DNA. The amplified DNA sequence can then be analysed by southern hybridization. i. Primer Construction: […]
Polymerase Chain Reaction: Steps, Advantages and Applications
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Prenatal Diagnosis of Genetic Diseases | Biochemistry
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The below mentioned article provides a study note on the Prenatal Diagnosis of Genetic Diseases. It is required to analyse DNA of family members of the affected individual to set up a diagnostic tool for a genetic disease. The markers (RFLPs) that are tightly linked to the disease is needed to be identified first. The […]
Amino-Acids Forming Acetyl-CoA
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Amino acids forming pyruvate are convertible to acetyl-CoA. In addition to this, 5 amino acids form acetyl-CoA directly, without first forming pyruvate. Phenylalanine: Phenylalanine (an essential amino acid) is converted to tyrosine by phenylalanine hydroxylase; tetrahydropteridine, NADPH and O, are required. The reaction is not reversible. The catabolism of phenylalanine and tyrosine may be discussed […]
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