The following points highlight the three main ways for confirming gene transfer. The ways are: 1. Marker and Reporter Gene 2. Southern Blotting or Southern Hybridization 3. Northern Hybridization. Way # 1.Marker and Reporter Gene: Transformation in eukaryotic system is a very low frequency event. It is, therefore, important that there should be some means […]
3 Main Ways for Confirming Gene Transfer
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6 Main Steps in Chromosome Methodology
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The following points highlight the six main steps in chromosome methodology. The steps are: 1. Pretreatment 2. Fixation 3. Hydrolysis 4. Processing 5. Staining 6. Mounting. Step # 1. Pretreatment: Pretreatment is an important step in the course of preparations of plant tissue for studying the chromosome number and their morphology in order to establish […]
Study Notes on Chromosome Technique
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The below mentioned article provides a study note on Chromosome Technique used in Plants:- 1. Study of Mitotic Chromosomes 2. Study of Meiotic Chromosomes 3. Identifying Features of Cytological Plates. Study of Mitotic Chromosomes: Mitosis occurs in all meristematic cells which are located in root tip, shoot tip, young leaf tip and other growing organs […]
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