In this article we will discuss about:- 1. Meaning of Stains 2. Purpose of Staining 3. Structural Components 4. Classification. Meaning of Stains: A stain is any colouring organic compound that, when combined with another substance, imparts a colour to that substance. The terms 'dyes' and 'stains' are often used interchangeably by biologists, but they are not the same. The [...]
In this article we will discuss about the staining procedure used for detecting bacteria. 1. Simple Staining Procedure: When a single staining-reagent is used and all cells and their structures stain in the same manner, the procedure is called simple staining procedure. This procedure is of two types - positive and negative (Fig. 17.5). In positive staining, the stain (e.g., [...]
The following points highlight the top five techniques for detection of bacterial pathogens. The techniques are: 1. Visual Separation of Infected Seed 2. Phage Method 3. Serological Techniques 4. Growing on Test 5. Indicator Test. Technique # 1. Visual Separation of Infected Seed: Parker and Dean (1968) found that bean seeds infected with Pseudomonas phaseollcola did not show external symptoms [...]