The below mentioned article provides a study note on gene sequencing. When a particular gene of interest or a DNA fragment is isolated, the sequencing of that piece of DNA becomes essential. The sequence of DNA refers to the order of nucleotide bases along its sugar phosphate backbone. No technique can determine the sequence of […]
Study Notes on Gene Sequencing | Genetics
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Top 3 Methods used in Gene Sequencing
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The following methods highlight the top three methods used in gene sequencing. The methods are: 1. Maxam & Gilbert’s Chemical Degradation Method 2. Sanger and Coulson’s Dideoxynucleotide Synthetic Method 3. Direct DNA Sequencing using PCR. 1. Maxam & Gilbert’s Chemical Degradation Method: In this method the following steps are involved (Fig. 18.17) i. The 3′ […]
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