In this article we will discuss about some physico-chemical principles which plays a great role in physiology with the help of suitable diagrams. Also learn the physiological importance of each physico-chemical principles. 1. Filtration: Filtration is the process by which un-dissolved particles are separated from a liquid through a membrane as a result of a mechanical force (filtering force). It [...]
Object: To separate the chlorophyll pigments by Thin Layer Chromatography (T.L.C.). Requirements: Spinach leaves, slides, staining tube, acetone, benzene, mortar, pastel, Kieselgel G, drier, pencil, sand, distilled water and oven. Preparation of T.L.C. Slides: 1. Prepare a slurry of Kieselgel by suspending about 2 gm. of Kieselgel in 10 ml. of distilled water. 2. Take 4 clean microscopic slides and [...]
Object: To demonstrate the phenomenon of plasmolysis. Requirements: Tradescantia leaf, water, sugar solution, slides, cover glass, microscope, blade. Method: 1. From the lower surface of the leaf of Tradescantia, peel off small segments of epidermis by a blade. 2. Put few peelings on a slide, mount in a drop of water, put a cover glass and study under microscope. 3. [...]