In this article we will discuss about some physico-chemical principles which plays a great role in physiology with the help of suitable diagrams. Also learn the physiological importance of each physico-chemical principles. 1. Filtration: Filtration is the process by which un-dissolved particles are separated from a liquid through a membrane as a result of a mechanical force (filtering force). It [...]
Object: To demonstrate the phenomenon of plasmolysis. Requirements: Tradescantia leaf, water, sugar solution, slides, cover glass, microscope, blade. Method: 1. From the lower surface of the leaf of Tradescantia, peel off small segments of epidermis by a blade. 2. Put few peelings on a slide, mount in a drop of water, put a cover glass and study under microscope. 3. [...]
Object: To separate leaf pigments by column chromatography. or To separate the chloroplast pigments by solvent method. Requirements: Pigments extract, magnesium oxide, filter glass disc, glass wool, flask, suction pump, chromatographic tube, petroleum ether, benzene. Method: 1. Take a chromatographic tube having a filter glass disc and glass wool at its base. 2. Fill the chromatographic tube with magnesium oxide. [...]