In this article we will discuss about the markers for chloroplast transformation. Generally, dominant antibiotic resistant genes are used in plastid transformation. Introduction of antibiotic resistant gene in the plastid genome facilitated the maintenance of high number of plastid genome copies in a cell. Chloroplast transformation invariably and unavoidably produces chimeric tissues, which requires identification […]
Markers for Chloroplast Transformation | Biotechnology
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Production of Selected Fatty Acids | Genetics
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Engineering crops for selected fatty acid production is one of the important aim for plant biotechnology. An attempt has been made to increase one particular fatty acid stearate in transgenic Brassica plant. Synthesis of high proportion of stearate (18: 0) can reduce the process of hydrogenation. Normally hydrogenation generates high amount of trans fatty acids, […]
Methods of Chloroplast Transformation in Plants
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The following points highlight the top five methods of chloroplast transformation in higher plants. The methods are: 1. Vectors for Plastid Transformation 2. Engineering for High-Level Protein Production 3. Biolystic Transfer 4. EG Mediated Transformation 5. Galistan Expansion Femtosyringe Method. Method # 1. Vectors for Plastid Transformation: Vectors used for plastid transformation utilize left (LTR) […]
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