In this article we will discus about the models for post-transcriptional gene silencing (PTGS). Production of dsRNA from inverted transgene repeats and single copy transgene triggers PTGS. It was proposed that dsRNA would be act as template for an RNA-directed RNA polymerase. Atleast four models have been proposed to explain induction of PTGS. First model […]
Models for Post-Transcriptional Gene Silencing (PTGS) | Genetics
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Mechanism of Antisense Ribosomes | Plants
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In this article we will discuss about the mechanism of antisense ribosome. Persistent search for novel vehicle for introducing antisense RNA into cells has become reality when Sweeney and Chaoyao (1998) developed antisense ribosomes in Tetrahymena thermophila. This system allows expression of antisense gene present with rRNA genes and antisense RNA alters expression function as […]
Examples of Molecular Markers | Plant Genetics
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Molecular markers are DNA sequences, whose inheritance pattern can be established. Classic examples of molecular markers are: 1. Restriction Fragment Length Polymorphism (RFLP) 2. Randomly Amplified Polymorphic DNA (RAPD) 3. Amplified Fragments Length Polymorphism (AFLP) 4. Sequence Characterized Amplified Region (SCAR). Example # 1. Restriction Fragment Length Polymorphism (RFLP): This novel non-PCR based approach is […]
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