In this article we will discuss about the tests for estimation of acid phosphatase in plants. Principle: The enzyme phosphatase hydrolyzes p-nitro-phenol phosphate. The released p-nitro-phenol is yellow in colour in alkaline medium and is measured at 405 nm. The optimum pH for acid and alkaline phosphatases are 5.3 and 10.5, respectively. Materials: (i) Sodium […]
Estimation of Acid Phosphatase | Enzyme Activity
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Estimation of Acetylcholine Esterase | Enzyme Activity
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In this article we will discuss about the tests for estimation of acetylcholine esterase in plants. Principle: The hydrolysed acetylcholine per unit time is measured by comparison of the initial concentration in a reference tube with the final concentration in the experimental tube. Acetylcholine is made to react with hydroxylamine to form the corresponding acylhydroxamic […]
Estimation of Proline | Plants
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In this article we will discuss about the tests for estimation of proline in plants. Principle: During selective extraction with aqueous sulphosalicylic acid, proteins are precipitated as a complex. Other interfering materials are also presumably removed by absorption to the protein-sulphosalicylic acid complex. The extracted proline is made to react with ninhydrin in acidic conditions […]
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