Tag Archives | Protoplast Culture

Protoplast Isolation: Sources and Methods | Biotechnology

In this article we will discuss about:- 1. Sources of Explant for Protoplast Isolation 2. Methods of Protoplast Isolation 3. Purification of Protoplasts 4. Isolation of Sub-Protoplasts 5. Protoplast Viability Test. Each plant cell has a definite cellulose wall which is attached with each other by middle lamella made up of pectin. Protoplast is the content which is surrounded only [...]

By |2016-11-02T05:49:29+00:00November 2, 2016|Biotechnology|Comments Off on Protoplast Isolation: Sources and Methods | Biotechnology

Top 8 Practical Applications of Protoplast Culture | Biotechnology

The following points highlight the top eight practical applications of protoplast culture. Application # 1. Production of Somatic Hybrids: To overcome the sexual incompatibility barriers the protoplast fusion programme may be taken to obtain the interspecific and inter-generic hybrids. For example, the fusion between Lycopersicon esculentum (tomato) and Solanum tuberosum (potato) have been possible to obtain the somatic hybrid 'Pomato', [...]

By |2016-11-02T05:49:28+00:00November 2, 2016|Protoplasts|Comments Off on Top 8 Practical Applications of Protoplast Culture | Biotechnology
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