In this article we will discuss about the Insertion of a Foreign DNA Fragment into a Vector. Introduction to the Insertion of a Foreign DNA Fragment into a Vector: The specific section of DNA generated by the action of restriction enzyme is spliced or incorporated into an agent called cloning vehicle. Cloning vehicle is also […]
Insertion of a Foreign DNA Fragment into a Vector
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Top 3 Methods of Recombinant DNA Formation
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The following points highlight the top three methods of recombinant DNA formation. The methods are: 1. Transformation 2. Transfection 3. Non-Bacterial Transformation. Recombinant DNA Formation: Method # 1. Transformation: The restriction enzyme which causes a break in foreign DNA also causes a staggered cut in the vector DNA at a specific cleavage site. The cohesive […]
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