Whole blood is diluted with a 3% acetic acid solution, which hemolyzes mature erythrocytes and facilitates leukocyte counting. The standard dilution for leukocyte counts is 1:20. This dilution is prepared using the leukocyte Unopette system. The dilution is mixed well and incubated to permit lysis of the erythrocytes. Following the incubation period, the dilution is […]
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