In this article we will discuss about the tests for estimation of free fatty acid. Principle: The free fatty acid in an oil is estimated by titrating it against KOH in the presence of phenolphthalein indicator. The acid number is defined as the mg KOH required to neutralize the free fatty acids present in 1 […]
Estimation of Free Fatty Acid
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Estimation of Nitrogen by Kjeldahl Method | Plants
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In this article we will discuss about the Kjeldahl method for the estimation of nitrogen in plants. Principle: Digestion of nitrogenous compounds by concentrated sulphuric acid in presence of catalysts (reduced iron powder, and a mixture of CuSO4 and K2SO4) produces ammonium compound. Further, such ammonium compound when distilled by steam in presence of strong […]
Estimation of Catalase | Enzyme Activity
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In this article you will discuss about the tests for estimation of catalase in plants. Principle: The enzyme catalase is responsible for catalysing the decomposition of H2O into H2O and O2. The assay of enzyme depends on estimation of residual H2O2 by titration with KMnO4. Requirements: (a) Reagents: 1. H2O2 —0.2 volume 2. H2SO4—10% 3. […]
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