Cloned genes can be introduced into knockout mice by means of embryonal stem cells (ES cells). Embryonal cells have attracted tremendous attention in recent years, hence a brief introduction on ES cells is warranted. The concept of ES cells arose through studies on mouse and human teratocarcinomas. These tumours were found to contain cells, called embryonal carcinoma cells, the stem [...]
In this article we will discuss about the role of Ti plasmids in genetic engineering of plants. Genetic engineering of plants is carried out by introducing DNA into a cell in culture that can grow into a mature plant. An efficient vector for introduction of recombinant DNA into plant cells has been developed from plant viruses. Furthermore, the Ti plasmid [...]
In this article we will discuss about the determination of DNA sequence. The sequence of bases A, T, G, and C in genes and whole genome can provide useful information for understanding gene structure and organisation, encoded protein and cell behaviour. The first complete nucleotide sequence of an entire viral genome (φX174) was determined in 1977 in the laboratory of [...]