Primary Cell Culture: 3 Techniques (With Diagram)

This article throws light upon the three types of technique used for primary cell culture. The three types of technique are: (1) Mechanical Disaggregation (2) Enzymatic Disaggregation and (3) Primary Explant Technique. Primary culture broadly involves the culturing techniques carried following the isolation of the cells, but before the first subculture. Primary cultures are usually prepared from large tissue masses. [...]