In this article we will discuss about:- 1. Basic Considerations of Nucleic Acid Hybridization 2. Hybridization Probes 3. Labeling of Hybridization Probes. Basic Considerations of Nucleic Acid Hybridization: Nucleic acid hybridization is a basic tool in molecular genetics which exploits the ability of single stranded nucleic acid molecules to hybridize with complementary sequences to form double stranded molecules. The principle [...]
Polymerase Chain Reaction-Oligonucleotide Ligation Assay (PCR-OLA) is a method to diagnose hereditary diseases caused by mutation not affecting restriction endonuclease sites. This method combines Polymerase Chain Reaction with the Ligation Assay. PCR-OLA distinguishes between the ligation and the absence of ligation of two oligonucleotides. A single nucleotide mismatch (at the site of hybridized oligonucleotides) prevents ligation and hence we can [...]
Just as the gene frequency is controlled in the genetic laboratory, so is the mating pattern, generation after generation. But outside the laboratory, chance mating is a chance or random affair. Every member of Mendelian population, thus, depends on chance or random matings. In other words, every male gamete in the gene pool has an equal opportunity of uniting with [...]