In this article we will discuss about the rapid gene regulation by auxin. Naturally occurring hormone indole acetic acid (IAA) is the fastest known hormonal action among plant in terms of rapid cell elongation within a short span of 10-20 minutes. Auxin exhibits two types of response. One is short-term effect with 20 min leads to cell elongation. Second is [...]
The following points highlight the nine main techniques of protoplast culture. The techniques are: 1. Agar Plating Method 2. Micro-Chamber 3. Nurse Culture 4. Immobilization of Protoplast 5. Nutrient Requirement 6. Growth Regulators 7. Cultural Conditions 8. Cell Wall Regeneration 9. Regeneration. Technique # 1. Agar Plating Method: Protoplast can be cultured conveniently by using agar plating method. Two millilitres [...]
In this article we will discuss about the significance of protoplast culture. Cell Modification by DNA Uptake: The isolated plant protoplasts are best suited for the introduction of foreign genetic material. Protoplast acts as an efficient receptor for the uptake of foreign DNA. Protoplast can be regenerated into the whole plant and this is indispensable for genetic manipulation. The ratio [...]