Exploitation of plant viruses as transformation vectors by massive infection may be harmful and even deleterious to the target plants. It is still however able to express and produce foreign proteins. Plant viruses must exhibit some of the exemplary features before they are considered as vectors. They should extend their broader host-range, spread of seed transmission and carry additional copies [...]
The following points highlight the eight main serological tests which helps in rapid diagnosis of plant viruses. The tests are: 1. Histochemical Test by Immunofluorescence 2. Agar Gel Double Diffusion Test 3. Direct Antigen-coated ELISA 4. Double Antibody Sandwich ELISA 5. Indirect Double Antibody Sandwich ELISA 6. Detection of Virus by Nucleic Acid Spot Hybridisation (NASH) using DNA Probe and [...]
Learn the comparison between virusoids and satellites. In 1981 for the first time J.W. Randles and coworkers discovered the virusoids. These are the small circular RNAs which are similar to viroids but they are always linked with larger molecules of the viral RNA. Robertson (1983) have reported that virusoids are essential for replication of the large RNA and, therefore, form [...]