In this article we will discuss about the top five techniques used for cloning of specific genes and their identification. The techniques are: 1. Direct Selection of Recombinant Clones 2. Colony Hybridization 3. Complementary DNA (c-DNA) 4. Isolation of a Specific Gene: Southern Blotting 5. Polymerase Chain Reaction (PCR). The restriction digest always contains an […]
Cloning of Specific Genes and their Identification
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Genome Library (With Diagram) | Microbiology
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A genome library consists of a large collection of bacterial cultures, in each of which a different segment of the genomic DNA of an organism has been cloned by recombinant DNA technology, so that the entire collection represents its complete genome. The analogy between a library of books and a genome library is based on […]
Classes of RNA Molecules and RNA Processing
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The following points highlight the three main classes of RNA molecules and RNA processing. The classes are: 1. Messenger RNA 2. Ribosomal-RNA 3. Transfer-RNA. Class # 1. Messenger RNA: Messenger RNA (m-RNA) is an informational molecule. The genetic message for protein synthesis contained in DNA is transcribed in the complementary ribonucleotide sequence of m-RNA. A […]
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