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Difference between Entamoeba histolytica and Entamoeba coli:

Difference # E. histolytica:

A. Trophozoite stage:

(i) Fairly abundant in amoebic dysentery stools.

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(ii) The size varies from 10-30µ

(iii) The outline shows finger like pseudopodia.

(iv) Actively motile in nature.

(v) Cytoplasm:

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Ectoplasm—prominent;

Endoplasm—finely granular with ingested RBCs

Vacuoles—scanty, spherical and defined.

Nucleus:

Nuclear membrane uniformly stained with chromatin; karyosome is central. In saline preparation the nucleus is indistinct and small.

B. Precystic stage:

The size varies from 5 to 15µ

C. Cystic stage:

(i) Size: 6-18µ

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(ii) Chromatid body present with rounded ends and they are bar like.

(iii) Usually one or four nuclei are seen.

(iv) Nucleus not visible when unstained.

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(v) Kidophilic vacuole—Prominent in uninucleate phase.

Difference # E. coli:

(i) Not abundant in stools.

(ii) 20-40 µ

(iii) Pseudopodia not prominent.

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(iv) Sluggishly motile.

(v) Ectoplasm—not prominent;

Endoplasm—coarsely granular loaded with bacteria, yeasts etc.

Vacuoles—often numerous, clefts and cracks are found.

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Nuclear membrane shows chromatin irregularly stained; karyosome eccentric. In saline preparation the nucleus is large and distinct.

12 to 17 µ

10 to 30 µ

Absent

Usually two or eight.

Visible when unstained.

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Largest in two—nucleate phase.